Detailed morphological analysis of axolotl sperm

The axolotl has extraordinary regeneration capacity compared to other vertebrates. This remarkable potential been attributed its life-long neoteny, characterized by the exhibition of embryonic characteristics at adult stage. A recent study provided a detailed morphological analysis sperm morphology Ambystoma mexicanum using routine and histological techniques. primary purpose present is describe simple inexpensive method for evaluating sperm. In this study, spermatophore structures were collected spread on slides air-dried. stained with periodic acid Schiff, toluidine blue, Masson’s trichrome, Giemsa, Spermac, Diff-Quik dye examination. coated gold/palladium scanning electron microscopy consisted an elongated head, neck, flagellum covered undulating membrane. lengths midpiece, tail, head 8.575 µm, 356.544 103.661 respectively. part, wavy membrane structure, whose function not explained, surrounds tail. data obtained from will constitute important step in designing future research reproductive axolotl.